In this context, we examined the ontogeny of peptidergic cell populations expressing Crh, Trh, and Ghrh mRNAs in the mouse hypothalamus, comparing their distribution relative to the major progenitor domains characterized by molecular markers such as Otp, Sim1, Dlx5, Arx, Gsh1, and Nkx2.1.
However, these genes had opposite expression profiles in the hypothalamus compared with the telencephalon (Table 1).
Microarray analysis of the hippocampus and hypothalamus comparing all treatment groups revealed no significantly-altered transcripts.
In mouse, hypothalamus showed a slightly increased median expression compared to other tissues (p = 0.05).
We then compared it to a LongSAGE library of mouse hypothalamus sequenced with the Sanger method.
Except for a slight tendency in mouse hypothalamus, imprinted genes do not show a particular tissue-specific enrichment compared to the genome-wide average in either adult tissues or mouse embryonic tissues.
In order to assess if we can properly explore the transcriptome complexity with this method, we compared this library with a LongSAGE library of mouse hypothalamus sequenced with the Sanger method.
In a recent study describing neuronal activity-dependent ribosome profiling in the adult mouse hypothalamus (Knight et al., 2012), Taf9b can be identified in the genomic data set as highly expressed in this tissue compared to several other TAFs.
Lin, D. et al. Functional identification of an aggression locus in the mouse hypothalamus.
Using Drop-seq, we have comprehensively profiled mouse hypothalamus under normal and food deprivation conditions (7).
