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We thank Julie Hanson and Michele Smart for mouse husbandry and mouse genotyping.
We thank Christophe Alberti, Yveline Bourgeois, Noura Mebirouk, and Josiane Ropers for assistance with mouse husbandry and handling and Mai Thao Bui and Elena Zinovieva for technical help.
Conditions of mouse husbandry and tissue collection and the breeding paradigm to generate sex-reversed and control mice were previously described [11].
Mouse husbandry and SI testing was performed as described for Experiment 2. Following 8 10 days of continuous housing in these social groups, all mice were socially isolated for 24 hrs and then tested for SI.
F.K.C. performed mouse husbandry and genotyping, qPCR and RT-qPCR.
Mouse husbandry and organ collection were approved by the Institutional Animal Care and Use Committee (ASP# 220-LG-2011).
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The authors are grateful to Vijay Yechoor, Shannon Hamilton, Karem Ozer, Karen Smith, and Taneasha Washington who assisted with teaching experimental techniques and mouse husbandry.
All animal experiments and mouse husbandry were done in the animal facility at the University of Salzburg in accordance with institutional and federal guidelines.
PlpCreERT; Nf1fl/ +; eGFP and PlpCreERT; Nf1fl/ fl; eGFP mouse husbandry, tamoxifen injections, tissue processing, histology, and electron microscopy were performed as previously described (Mayes et al., 2011).
The VelociMouse® method provides several unique opportunities for shortening project timelines and reducing mouse husbandry costs.
Our own laboratory personnel carried out all aspects of the mouse husbandry under strict guidelines to insure gentle and consistent handling of the mice.
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