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We thank Daniel Baizan, Cristina Pintos, and Maria Clemente for mouse husbandry.
The VelociMouse® method provides several unique opportunities for shortening project timelines and reducing mouse husbandry costs.
Students will have the opportunity to gain training in evolutionary biology, experimental design, CT scanning, and mouse husbandry.
Students will have the opportunity to gain training in evolutionary biology and genetics, experimental design, mouse husbandry, and molecular biological techniques.
We thank K. Merkle for machining the various custom parts of the microscope, S. Sinha for help with coating miniature mirrors, and J. Li for help with histology and mouse husbandry and genotyping.
While the power of this approach is indisputable, the effort necessary for its success remains substantial, requiring careful attention to aspects including ENU treatment, mouse husbandry, screen assay design, genetic mapping, positional cloning, and mutation validation.
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We are indebted to Rose Madeira and Anita Hawkins for their assistance with mouse breeding and husbandry, to Gunjan Gilbert, Kathryn Sawyer and Caleb Dresser for technical help and assistance in mapping, and to Ken Paigen for critical reading and helpful comments.
Young and old mouse strain and husbandry conditions as described in (Miller et al., 2011).
Mouse handling and husbandry followed the regulations established in the UK Home Office's Animals (Scientific Procedures) Act 1986.
A.F. performed and analyzed the retina and zebrafish experiments, A.L. and C. Raimondi performed and analyzed the cell culture experiments, G.G. performed zebrafish experiments, V.S. performed mouse genotyping and husbandry, I.Z.
Environmental variation that cannot be readily controlled in humans can be minimized in mice by consistent husbandry in a stable laboratory colony.
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