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c Light microscope image of a resin-embedded BACE1+/+ mouse hippocampal section that underwent pre-embedding silver-enhanced immunogold labeling for BACE1, showing the same pattern of immunoreactivity as in a. d Silver-intensified BACE1 immunogold signal is lacking in BACE1−/− hippocampus.
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Mouse hippocampal sections were prepared and stained with H&E (haematoxylin and eosin) as described previously [ 15].
Watanabe, S. et al. Ultrafast endocytosis at mouse hippocampal synapses.
Marciniak, E. et al. The chemokine MIP-1α/CCL3 impairs mouse hippocampal synaptic transmission, plasticity and memory.
Here whole-cell recordings were performed from synaptically connected pairs of neurons in mouse hippocampal slices from PV-GFP mice [1].
Note the almost absence of immunoreactive B cells in the EB102-treated mouse brain sections (k), whereas the hippocampal sections of the EB101-immunized mice (j) show a moderate immunoreactivity in response to the neuropathological pro-inflammation process.
(d) Hippocampal sections of KO mice stained with HE.
(E ) EM48 immunostaining of hippocampal sections from Q171-N82 mice at the age of 15 weeks after 1 month of treatment with vehicle or AMD3100 at 10 mg/kg body weight daily by intraperitoneal injection.
Taking advantage of GFP immunofluorescence, we examined localization of the different MeCP2 variants in hippocampal sections of hemizygous male mouse brains (Fig. 4C).
For a first characterization of GFP-labeled interneurons in GAD65-GFP mice, we performed immunohistochemistry for the inhibitory neurotransmitter GABA and the GABA-producing enzyme GAD67 in hippocampal sections of adult GAD65-GFP mice.
As a negative control to demonstrate antibody specificity, hippocampal sections from BACE1−/− mice treated with the anti-BACE1 antibody lacked BACE1 immunoreactivity using either the immunofluorescence or immunogold staining protocol (Suppl. Fig. 1b, d).
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