Total RNAs were isolated from mouse hepatic tissues using the RNeasy (QIAGEN), from which cDNAs were synthesized from total RNAs with the Superscript First-Strand Synthesis System and random hexamer primers.
In conclusion, a deficiency of eNOS-derived NO may change the fat distributions in the liver and viscera, thereby promoting the progression of disease in an HFD-induced, early-stage NASH mouse model by changing the hepatic tissue blood flow.
As shown in Fig. 4, in comparison with the normal cellular architecture of hepatic tissue of mice in the NC group (Fig. 4a), extensive liver damage in diabetic mice were observed after the injection of STZ characterized by cellular degeneration, hepatocyte necrosis, and lipid droplet accumulation (Fig. 4b).
In our eNOS-knockout mouse model, while wild-type mice fed an HFD had a significantly lower hepatic tissue blood flow than the wild-type mice fed a BD, the eNOS-knockout mice fed a BD had a significantly lower hepatic blood flow than the wild-type mice fed a BD.
While systemic insulin resistance was comparable between the eNOS-knockout and wild-type mice fed a high-fat diet, hepatic tissue blood flow was significantly suppressed in the eNOS-knockout mice, compared with the wild-type mice, in mice fed a high-fat diet.
(A ) Venn diagram showing overlap of increased and decreased genes with sets of genes similarly altered in hepatic tissue of CR-fed mice, mice with a liver-specific Keap-1 mutation, and long-lived Ames dwarf mice.
Total mRNA isolated from murine hepatic tissue in mice treated with APAP alone, IFN-β alone or a combination of IFN-β+APAP were compared to those of mice treated with Vehicle alone at 1.5 and 4 hours post-treatment.
This hypothesis was supported by our analyses and we show that "signature transcripts" highly expressed in certain leukocyte populations (e.g., thioglycollate-elicited peritoneal Mφ) are often overwhelmingly elevated in hepatic tissue of mice provided the high fat diet.
These results indicate that high expression of Irf5 (and other genes associated with TLR signaling) is a common characteristic of cell populations that appear to infiltrate hepatic tissue in mice provided a HF diet.
