Sentence examples for mouse hearts using from inspiring English sources
The manipulation of miR levels in mouse hearts using genetic techniques or engineered miR mimetics and antagonists is elucidating the roles of specific cardiac miRs in cardiac development, and in the cardiac response to injury or stress, and heart disease.
Total RNA was isolated from transgenic mouse hearts using Trizole (Invitrogen, USA) according to the manufacturer's protocol.
Total histone fractions were isolated from pooled (n = 6) neonatal (P3 5) Smyd2flor/flox or Smyd2 conditional knockout (cKO) mouse hearts using the EpiQuick™ Total Histone Extraction Kit (Epigentek) according to the manufacturers protocol.
Briefly, cardiomyocytes were prepared from mouse hearts using standard protocols [52] and plated immediately on chamber slides and fixed in 4% paraformaldehyde in PBS for 20 minutes at room temperature and stored in 70% ethanol at 4°C.
Total RNA was extracted from mouse hearts using "RNeasy® fibrous tissue kit" (Qiagen).
Total RNA was isolated from mouse hearts using the Trizol reagents (Invitrogen) and from BAECs using the RNase easy mini kit (Qiagen) following the manufacturers' instructions.
In the present report we have optimized the bioenergetics assay in mitochondria isolated from mouse heart using XF analyzer, and determined the effect of age on mitochondrial function.
Here, we undertake a systematic morphological and molecular evaluation of Cre-recombinase induction in the mouse heart, using standard reagents and protocols, and present a protocol that avoids these pathological responses.
Nuclear extracts were prepared from freshly isolated wt and Nox4-overexpressing tg mice hearts using the NE-PER Nuclear and Cytoplasmic Extraction kit (Pierce Biotechnology) as per the manufacturer's recommendations.
The RCR for mouse heart mitochondria using succinate as substrate was 4.087 for young mice heart and 3.76 for old mice heart using our isolation procedure which is similar to Rogers et al. (2011) with some modifications.
It was determined that the basal OCR of young mice was significantly higher than the older mice heart using 1 µg mitochondrial protein (Fig. 2A B).
