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We calculated z-scores for all matrices for the following 22 gene lists: 167 selected CCGs, all 2065 CCGs, 4 tissue-specific CCG lists (mouse heart, liver, SCN, skeletal muscle) and 16 tissue- and phase-specific lists of CCGs.
We tracked the expression of Cdkl5 in mouse heart, liver and kidney over development from embryonic day 11 to postnatal day 30.
The only acute sidestream study investigated oxidative DNA damage in mouse heart, liver and lung tissue after a single CS exposure [ 24].
Howard et al. [ 24] investigated oxidative DNA damage via analysis of 8-OHdG (8-hydroxy-2′-deoxyguanosine) in mouse heart, liver and lung tissue and detected increased levels already after a single CS exposure for 30 min.
For example, Nord et al. [ 31] recently collected genome-wide H3K27ac modification profiles from seven developmental stages (starting with E11.5 through adulthood) for mouse heart, liver, and forebrain tissues and found rapid and pervasive turnover in the H3K27ac modification landscape across the development of these tissues.
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Enrichment of the putative novel transcripts in testes and liver was further examined experimentally using mouse heart, testes, liver, kidney, brain and lung tissues by qRT-PCR and RT-PCR, to determine the expression levels and patterns.
Duplex Doppler provides blood velocity sensitivity to the mm per s range, consistent with flow in the microcirculation, and can readily detect blood flow in the embryonic mouse heart, aorta, liver and placenta.
The cDNAs were produced with a First Strand cDNA Synthesis Kit (MBI Fermentas, Heidelberg, Germany) using total RNA isolated from mouse heart and liver samples.
Semi-quantitative immunoblot analyses were carried out in mouse heart and liver tissues as described previously (Collino et al., 2006).
Total proteins were extracted from mouse heart and liver, separated by SDS/PAGE and transferred to PVDF membranes (GE Healthcare).
Briefly, mouse heart and liver samples were homogenized in 10% homogenization buffer and centrifuged at 1323 g for 5 minutes at 4°C.
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