Sentence examples for mouse heart extracts from inspiring English sources
It is not known if acute pulmonary zinc exposure inhibits cardiac aconitase activity; however, zinc transfer from metal-lothionein to cardiac mitochondrial aconitase has been noted in mouse heart extracts (Feng et al. 2005).
As previously reported [5], U1013 anti-mXin antibody recognizes a major band mXinα (155 kDa) and its splicing variant mXinα-a (250 kDa) as well as a 340 kDa mXinβ band in mouse heart extract (Fig. 4).
RNA from mice heart was extracted by RNeasy Fibrous Tissue Mini Kit (Qiagen).
Whilst the cDNA prepared from positive control EMC virus RNA, amplified with both primer sets, the cDNA prepared from RNA extracted from mouse heart samples failed to amplify with either of the Cardiovirus primer sets.
Total proteins were extracted from mouse heart and liver, separated by SDS/PAGE and transferred to PVDF membranes (GE Healthcare).
Mouse heart homogenates, microsomal, MAMs and mitochondria extracts were obtained by protein fractionation.
PDE4 localizes to the Z-band of cardiomyocytes in human (Fig. 1c) and mouse heart (data not shown), suggesting that PDE4 activity detected in heart extracts is due to the expression of PDE4 in the cardiomyocytes and that PDE4 acts in defined subcellular compartments of these cells.
β-gal was determined in heart extracts from young and old mice using the soluble β-galactosidase method.
Total heart extracts (10 µg) from E9.5 and E12.5 mice were subjected to 5% SDS-PAGE and analyzed by western blotting with anti-IP3R1 (18A10) [35] and anti-IP3R2 (KM1083) antibodies [36].
Total RNA was extracted from P7, P28, P53, differentiated cells, and whole mouse heart tissue using TriZol reagent (Invitrogen), according to the manufacturer's instructions.
Western blot analysis of whole heart extracts and IF staining of isolated adult myocytes with sera derived from diseased Stat3C/C mice showed strong reactivity against proteins of the heart but not of other tissues including skeletal muscle, suggesting auto-immunity against heart components (Fig 4A and B).
