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The estimate of the number of unique transcripts in the adult mouse heart derived from the extrapolation of the results of SAGE experiments exceeds 23,000 [ 37], and a similar order of magnitude has been suggested for human heart [ 39].
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These data indicate that the mouse heart-derived Sca-1+ cells isolated here represent a Sca-1+/CD31 subpopulation.
We have isolated postnatal mouse heart-derived Sca-1+/CD31 CPCs by MACS, which can be propagated in vitro over the long-term while retaining stemness characteristics and multiple differentiation potentials.
Overall, these data indicate that mouse heart-derived Sca-1+ cells are multipotent, retaining an ability to differentiate into different cardiac cell lineages, despite long-term propagation in vitro.
Mouse bone marrow derived macrophages (BMDMs) were isolated and cultured using the following procedure59.
Jones et al. initially demonstrated Grb7 and Grb14 interactions with Tie2 in a yeast two-hybrid screen using cDNAs derived from embryonic mouse heart and lung tissue [ 5].
Based on studies of human and mouse heart development and mESCs, efforts were undertaken to derive cardiac progenitor cells from hESCs.
This is in accordance with results from SAGE analysis of the adult mouse heart transcriptome, which indicate that the cardiac tissue contains the highest percentage of mitochondrial-genome derived transcripts [ 37, 38].
A whole mouse heart from mice were excised and washed in cold PBS and the cytosolic and mitochondria fractions were derived following the Mitochondrial/Cytosol Fractionation Kit manufacturer's protocol (DBI Bioscience).
During both zebrafish and neonatal mouse heart regeneration, differentiated CMs re-enter the cell cycle to proliferate and genetic lineage tracing experiments indicate that the majority of the newly forming myocardium is derived from pre-existing CMs [ 13– 15].
Fetal mouse heart in organ structure: ultrastructure.
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