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Mouse heart data were downloaded from NCBI accession GSE36025, sample SRR453174.
PDE4 localizes to the Z-band of cardiomyocytes in human (Fig. 1c) and mouse heart (data not shown), suggesting that PDE4 activity detected in heart extracts is due to the expression of PDE4 in the cardiomyocytes and that PDE4 acts in defined subcellular compartments of these cells.
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When clofilium was applied, the beating of the mouse heart ceased (data not shown).
In comparison, 1 mM NaCN was sufficient to block succinate-supported respiration in freeze-thawed mouse heart mitochondria (data not shown).
Furthermore, due to the small size of the mouse heart, technical limitations prevented us from recording endocardial data.
All data were obtained during daylight hours, when the mouse heart rate is more stable than during the more active nocturnal hours.
In this section, I evaluate the ability of mouse H3K4me1 and H3K27ac data from different stages of heart development to predict validated mouse heart enhancers at E11.5.
All data was obtained during daylight hours, at which point the mouse heart rate is stable before increasing during the more active evening/night hours.
In this paper, we presented the first quantitative data on the number of blood islands at various stages of mouse heart development.
The authors present data indicating that newt and zebrafish heart muscle cells contain centrosomes, whereas adult mouse heart cells do not.
In the present study, our data for the first time showed that Rnf207 was highly expressed only in the mouse heart.
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