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Blood samples from all treated and control mouse groups were collected and centrifuged to obtain plasma samples.
Methods: Three experimental mouse groups were formed: PBS + IgG (IgG instillation after PBS exposure), LPS + IgG (IgG instillation after LPS exposure), and LPS+ EphA2 mAb (EphA2 monoclonal antibody instillation posttreatment after LPS exposure).
Both mouse groups were carefully controlled for anatomical and physiological parameters relevant for edema formation and stroke outcome.
For determination of lung viral titers, mucosal immune responses, and cytokine ELISPOT and memory B cell assays, additional sets of mice were intranasally immunized with H5N1 VLPs containing 0.3 µg H5 HA, and some groups of these mouse groups were intranasally administered with 5 µg of inactivated reassortant rgΔH5N1 virus 6 days earlier prior to euthanasia and organ sample collection.
All mouse groups were sacrificed on day 40.
The mouse groups were kept under observation for 3 weeks after the drug treatment.
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H&E-stained images from lungs of the corresponding mouse groups are shown in the bottom left panels.
Then, the rate of a certain hair morphogenesis stage in the total hair follicles and the median score in each mouse group were determined.
Then, the rate of a certain hair cycle stage in the total hair follicles and the median score in each mouse group were determined.
A negative control mouse group (N = 30) and a sham-infected mouse group were also included in the experiments.
Half of the crypts of each mouse group were cultured in crypt culture medium supplemented with 0.1 μg/ml 4OHT.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com