It was interesting to note that the relapse rate did not differ among mouse groups receiving Std-Rx alore or those receiving it in addition to tofacitinib.
The cells (10×106, 25×106, and 50×106) in 100 µl of Media (Isolyte S, pH 7.4) were delivered over 5 min. The Media mouse group received 100 µl of Isolate S, the same volume administered to cell transplant mice.
A second mouse group received a control diet (C1000, both from Altromin, Germany).
The WD ApoE-/- mouse group received Teklad Adjusted Calorie Diet (42% from fat) (Harlan Catalog # 88137; 0.2% cholesterol) ad libitum, a diet that accelerates atherosclerosis in ApoE-/ mice [ 59– 62].
After DMN injections, each mouse group received oral administration of β-sitosterol, which appropriately diluted with DW, or saline for two weeks.
All mice groups received water ad libitum.
NKB administered mice groups received low, medium, high doses of 100, 200 and 400 mg/kg, respectively.
In comparison, the mouse group receiving oral daily administration exhibited more dose-dependent effects, and the pharmacological effect diminished rapidly in conjunction with a reduction of the plasma drug levels 8 h after the last administration of isoperidone on day 3.
The statistical analysis performed using Fisher's exact test showed that the difference in TFF between the mouse group receiving 3 MBq/mL of 213Bi-MX35 and the control group and between the group receiving 9 MBq/mL of 213Bi-MX35, and the control group was statistically significant (p = 0.02) and (p = 0.0002) respectively.
As shown in Fig. 4, a significant reduction in tissue bacterial loads were observed in the mouse group receiving iCD8+ DC compared with the mice without DC transfer (sham treated control mice).
