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Mouse groups consisted of twenty mice.
The four mouse groups consisted of the LPS-only group (n = 5), the LPS with 0.1 mg/kg Bev group (n = 10), the LPS with 1.0 mg/kg Bev group (n = 10), and normal-saline-only group (negative control, n = 5).
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Female C57/BL6 mice were individually identified with ear tags and randomly assigned to three experimental groups containing eight mice per group: groups consisted of mice to be infected with either Y. enterocolitica strain 8081, strain WA-314 or both in combination (Table 4).
Control groups consisted of untreated mice and mice treated with vehicle alone (PBS).
Control groups consisted of untreated mice or mice treated with pVAX1 empty vector.
All study groups consisted of mice from at least 14 different litters.
The groups consisted of mice fed 0.0002, 0.002, 0.02, and 20 μg LT-B per pellet per feeding and two groups fed nt maize.
Groups consisted of mice receiving saline (0.9% NaCl {pH 7.4}), APMS-MB (no DOX, DOXX high (0.2 mg/kg), DOX low (0.05 mg/kg), or APMS-MB-DOX (0.05 mg/kg) (n = 6 mice/treatment group).
The two experimental groups consisted of mice where MyD88 was present in the CNS but not in bone marrow-derived cells (i.e. KO→WT; Group 1) and vice versa (i.e. WT→KO; Group 2), whereas bone marrow transfers into genetically identical mice (i.e. KO→KO and WT→WT; Groups 3 and 4 respectively) were also performed to rule out any non-specific effects of ionizing radiation on the responses obtained.
Experimental groups consisted of fetal mouse hearts taken from fetuses killed prenatally at 11.5, 12, 12.5, 12.75, 13.0, 14.0, 14.0 dpc.
NPCB groups consisted of 8 mice for each dose and time point; vehicle control groups included 12 mice for each time point.
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