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In contrast, mice that received one dose of rEF-Tu s.c. and both mouse groups boosted mucosally (s.c. + i.n. and i.n. + i.n).n
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Most of the smaller groups boosted Kihuen.
At the second and fourth weeks after the first immunization, all mice groups were boosted with the same plasmid dose i.m.
Although two of the orally boosted mice were not able to respond to preparation, the response pattern was similar to that observed for reference group boosted with Engerix B. When mice were orally delivered with the control tissue, no boosting effect was observed.
Titre of anti-HBs antibodies in mice boosted orally with lyophilised powder reached a mean value of 293 mIU/mL, while in the group boosted by injection with Engerix B they reached 397 mIU/mL.
Twenty-one days after primary immunization, some groups of mice were boosted in the same way with 200 μg of CII in 0.05 M acetic acid, emulsified in incomplete Freund's adjuvant (IFA).
One month later, during consecutive days (30, 31, 32 days), each group of mice was boosted intramuscularly (i.m).
Each group of mice was boosted on day 14 with identical formulations, and serum was collected over time for analysis of titers of anti-OVA IgG.
Three months later, one mouse was boosted without adjuvant intraperitoneally 4 days prior to the fusion.
The researchers then took a group of neurologically compromised mice and boosted their levels of RBM3.
Two additional groups were included: one of them was vaccinated as SAG1 group and two weeks later mice were boosted intradermally (ID) with 0.4 μg of rSAG1 expressed in E. coli emulsified in IFA (SAG + boost group); the other group was injected as the Control group and boosted ID with 0.4 μg rSAG1 + IFA Controll + boost group).
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