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Ileitis was also observed in a PI3K+/−SHIP−/− mouse (grade 4), indicating that a third independent SHIP mutant strain develops this pathology and that PI3K haploinsufficiency does not protect from development of ileitis.
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Female ICR mice (Grade II, 5 weeks old) weighing 18 22 g were purchased from Zhejiang Experimental Animal Center (Certificate No. SCXK 2008‐0033, Hangzhou, China), and acclimatized for 1 week prior to use.
The ApoPep-1 probe facilitates the detection of OA as early as 2 weeks after DMM in mouse models (grade II OA) by specifically detecting apoptotic chondrocytes within 30 minutes.
Although the number of seizures was low, all Tg20 mice showed Grade VI seizures, while Prnp −/− mice showed Grade V-VI.
These heterogeneous neoplasms were clinically characterized as genetically engineered mouse schwannomas, grades II and III.
The following growth factors were also included: 20 ng/ml purified mouse receptor-grade epidermal growth factor (BD Biosciences, Sydney, Australia) and 10 ng/ml recombinant bovine basic fibroblast growth factor (Roche, Basel, Switzerland).
Five of the 53 (9%) of SHIP-deficient mice had grade 3 inflammatory lesions within the ileum.
In C57Bl/6 mice, clinical grade bleomycin sulfate (Blenoxane; Bristol-Myers Squibb, NYw York, NY, USA) was utilized.
Human A549 tumor models were established in 6 – 8 weeks old, athymic nude mice (SPF grade).
The ileum of 9 of the 53 (17%) of the SHIP-deficient mice had grade 1 lesions.
Six of the 53 (11%) of SHIP-deficient mice had grade 4 inflammatory lesions within the ileum.
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