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In our study we used PDGF-B driven PTEN-deficient and PTEN-intact mouse gliomas generated in nestin-tv-a/ink4a-arf-/-/ptenfl/fl mice.
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We generated mouse glioma using the RCAS/tv-a system for driving PDGF-BB expression in a cell-specific manner.
However, mouse gliomas show high expression of miR-21.
GL26 mouse glioma cells were kindly provided by Prof. L. Zitvogel (Institut G. Roussy, Paris, France).
In this case, the ultrasound-sensitive gas-cored liposomes carried the siRNA-loaded micelles, resulting in enhanced delivery efficiency and gene silencing in a mouse glioma model.
To determine if IL-17A mRNA is increased in mouse glioma, normal mouse brain, PBS-injected WT mouse brain, WT mouse brain with glioma and Rag1−/− (lack functional T and B cells) mouse brain with glioma were analyzed (Fig. 2A).
To determine if Th17 cells develop in glioma-draining lymph node (dLN) and are present in mouse glioma, PBS-dLN, glioma-dLN, PBS-brain and glioma-brain were analyzed at 3 weeks post-operative in WT mice.
Collectively, the results suggest that Th17 cells infiltrate human and mouse glioma.
In a mouse glioma model, Bmi1 was implicated in tumorigenesis in an Ink4a/Arf-independent manner [23].
Furthermore, the presence of Th17 cells was confirmed in both human and mouse glioma.
It is also demonstrated in mouse glioma models that normal progenitor or stem cells can be recruited to glioma niche [45].
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