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Because of its quantitative precision, specificity, and compatibility with high throughput robotic operations, the LOA assay eliminates bottlenecks in ES cell screening and mouse genotyping and facilitates maximal speed and throughput for knockout mouse production.
We wish to thank Martine Lessard for Cp mouse genotyping and Nathalie Laflamme and Paul Prefontaine for technical assistance.
We thank Dr. Pastor-Anglada M. (University of Barcelona, Spain) for human ENT1 expressing construct, S. Choi for mouse genotyping and Western blot analysis, D. Drubach for managing clinical data, S. Hammer for consulting for human DNA sequencing and genotyping, and D. Frederixon for proofreading of the manuscript.
VLM, CCI, SD performed mouse genotyping and blinding for electrophysiology.
Details on mouse genotyping and handling can be found in the Extended Experimental Procedures.
A.F. performed and analyzed the retina and zebrafish experiments, A.L. and C. Raimondi performed and analyzed the cell culture experiments, G.G. performed zebrafish experiments, V.S. performed mouse genotyping and husbandry, I.Z.
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Each session was videotaped and videos were analyzed by pre-trained investigators blind to the test mouse genotype and treatment.
The slides were coverslipped with Vectashield (Vector Laboratories) and examined under an Olympus BX60 epifluorescence microscope by an assistant blinded to mouse genotype and age.
Images were acquired and analysed by investigators blinded to mouse genotype and treatment.
All assessment and analysis was performed by an experimenter blind to mouse genotype and treatment.
All scoring was conducted by an experimenter blind to mouse genotype and treatment.
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