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Mouse genotypes were confirmed by PCR using genomic DNA extracted from tails or yolk sacs.
Individual mouse genotypes were scored using established agarose gel electrophoresis protocols.
Mouse genotypes were determined by polymerase chain reaction analysis as previously reports [16], [18].
Mouse genotypes were identified using genomic PCR with primers TNK1S2F, NEO1 and TNK1S2R.
Mouse genotypes were confirmed either by hemoglobin gel electrophoresis or by PCR.
The mouse genotypes were determined by PCR, using tail DNA as the template and the primers (E1: 5'-CACATTGATTTCTTTGTGCCTG-3' and E2; 5'-TCTGTACAATCATCCTGCAG-3'), which anneal to the loxP flanking regions.
Similar(48)
A critical and consistent difference between the two mouse genotypes is the earlier disruption of the BBB and the concomitant hemorrhagic lesions in H3R−/− mice at day 3 post-infection, a time when no clinical sign had manifested.
Mouse genotypes are h SMN2+/+; mSmn+/+or h SMN2+/+ and mSmn−/−.
In some mice, genotypes were reconfirmed at the protein level by Western blotting (Fig. 1B).
Mouse genotyping was performed from tail biopsies.
Mouse genotyping was done by PCR and sequencing of tail-snips using the Mouse Genotyping Kit (KAPA Biosystems) and primers listed in Table S2.
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