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Western blot analysis did not show significant differences in Gapdh levels in the mouse genotypes studied (Fig. S8).
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No defects of the mandible were seen in mice of any genotype studied.
For UUO, all parameters investigated did not differ between contralateral kidneys and kidneys from control mice (without surgery) in each genotype studied (data not shown).
D.E.K. has served as a consultant for Abbott Laboratories and Gilead Sciences, Inc. DS performed the arterial pressure and Na balance studies, SR performed the RNA analysis, SW performed the DNA analysis, RK developed and provided the Pax8-rtTA and LC-1 mice, KAS supervised the breeding and analysis of mouse genotypes, and DEK designed the study, analyzed the data, and supervised the project.
We next studied the radio-sensitivity difference between different mouse genotypes.
Finally, the variation among B6, BR and C3H mice was studied by analyzing genotypes at known single nucleotide polymorphisms (SNPs) and sequencing non-coding regions of genes to generate haplotype maps of the identified region.
For quantitative evaluation of CD115 and F4/80 staining, positive cells were counted manually in the nerve ganglia in a blinded study (reviewer blinded to the mouse genotype).
Male and female mice of each genotype were studied as shown in Table 1.
See Table 1 for PC mouse genotype.
Mouse genotyping was done by PCR and sequencing of tail-snips using the Mouse Genotyping Kit (KAPA Biosystems) and primers listed in Table S2.
Routine mouse genotyping was performed by PCR.
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