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Exact(29)
This dataset consists of 68 mouse genomic sequences and their top homologs from the human genome.
In addition to homology search in the bovine genome sequence, ETS were applied to similarity search against human and mouse genomic sequences of the NCBI database [genome (all assemblies)] [ 62, 63] using the cross-species MegaBLAST tool.
Previous transcriptomic studies which relied on cross-hybridization to mouse DNA microarrays have showed considerable success [ 72] and in general, results from alignment to both mouse and rat reference genomes suggest that CHO genomic sequences are generally more similar to mouse genomic sequences [ 73].
In general, results from alignment to both mouse and rat reference genomes suggest that CHO genomic sequences are generally more similar to mouse genomic sequences, as previously demonstrated [ 15]. * Pseudogenes, RNA genes, and genes on Y chromosome were excluded from this analysis.
Our program was tested on two different benchmarks that include several pairs of real human and mouse genomic sequences.
On a benchmark including 120 pairs of human and mouse genomic sequences, most of their encoded genes were successfully identified by our program.
Similar(31)
These results are consistent with previous reports that HS sites are i-enriched in human genome regions that align with mouse genomic sequence [ 17] and ii-often located near sequences conserved in multiple species [ 18].
The completion of the mouse genomic sequence and progress towards sequencing the rat genome in the past year will enable the molecular identification of quantitative trait loci and induced mutations.
These two putative cDNAs are unspliced, lack major polyadenylation signals, and end in a stretch of polyA mouse genomic sequence, suggesting that they may represent genomic DNA contaminants.
For this, we cloned a library of fragments from a mouse genomic sequence in a lentiviral vector, next to a minimal promoter-reporter cassette.
Mouse genomic sequence, encompassing loci plus 10kb each of upstream and downstream sequence, was screened for pairs of FoxH1 binding sites within 30 200 bps of each other; all possible sequence orientations were tested (as described in the Materials and Methods).
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