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We examined for syntenic conservation between the human and mouse genomes (see Methods for details).
Those CAGE-defined promoters that were not mapped to known orthologous regions were then mapped to the whole RepeatMasked target genome (pig or mouse) using blastn to find the best match (pig or mouse genomes) (see methods and Additional file 1: Figure S1).
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to the presence of a Xiap-like sequence found on mouse chromosome 7 during sequencing of the mouse genome (see: http://apr2006.archive.ensembl.org/Mus_musculus/domainview?domainentry=IPR001370).
The genome-wide threshold for significance for transgene insertion site scores is calculated by random alignments to the reference mouse genome (see Materials and Methods).
We detected breakpoints on the human genome using the software Cassis [ 7], and grouped them by pairs such that each distant pair in the human genome corresponds to adjacent loci in the mouse genome (see Figure 1 and Methods).
By developing a local alignment-based procedure with the new measurement, we analyzed the conservation of 1642 human functional regions from the ENCyclopedia Of DNA Elements (ENCODE) project [ 12] in the mouse genome (see Methods).
In this report, we show that the combination of both the in silico prediction and the in vivo data leads to the discovery of many new putatively coding sORFs in the mouse genome (see Additional file 2).
Specifically, we noted that a certain DNA sequence designated H4.141 (5'-AGGCTGaAGTGCA-3') was > 100-fold overrepresented compared to other HNF4α binding sites in the human, but not the mouse, genome (see additional file 1: Figure S1).
To produce an accurate domain alignment, we performed phylogenetic analyses of the individual FN3 and IG domains present in the neogenin protogenin proteins of the mouse genome (see Supplementary Material online for more details).
a For a more detailed survey of domesticated TEs in the human genome see Additional file 1 a For a more detailed survey of domesticated TEs in the mouse genome see Additional file 2 Using these selection criteria, we identified a total of 63 transcribed protein-coding genes within the human genome derived from previously characterised TEs.
We developed a software package (also named REDseq; available as a Bioconductor package) to assign each read to a unique RS in the mouse genome (see Methods for details), and count the relative cut frequency per site corresponding to normalized read counts assigned to each RS.
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