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B6.D2 mice have a genomic insert on chromosome 12 from the DBA/2J mouse genome; this insert spans 35.4 41.0 Mbp and contains 15 genes.
We removed 6808 probe sets from the analysis because these map to multiple locations on the mouse genome; this resulted in 38293 probe sets for further analysis.
Together with recent advances in the manipulation of the mouse genome, this information has established the mouse as the premier organism for developing models of human disease and drug action (2).
Students are asked to finish their project to the same standard as the mouse genome; this requires 1) a complete assembly (no gaps), 2) adequate coverage of all regions (ideally, sequence from both strands), 3) resolution of ambiguous results, and 3) high quality of sequence data (Phred score of >30; The Genome Center at Washington University, 2004).
Thus, in 1994, Gurney and colleagues created the SOD1 G93A transgenic mouse line [Tg SOD1*G93A 1Gur], which carries a mutant human SOD1 cDNA inserted randomly into the mouse genome; this line remains the most widely used model of human ALS (Gurney et al., 1994).
To protect the activity of the 8GBS-hsp68-eGFP from position effects when integrated into the mouse genome, this fragment was then subcloned in the BamH1 site of pJC13-1(Chung et al., 1993 ), which contains 2 insulators on each side of the BamH1 site.
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In the current study, we chose the model of APP/PS1 double Tg mice, which were generated by knocking identified familial AD and/or PS1 into the mice genome; this model has been widely adopted by other scientists.
In the mouse genome, this gene contains 6 introns, where they are again located in the 5' UTR.
We blasted the mouse genome with this partial sequence and found a 344-bp region and a 379-bp region that exhibit sequence homology (69% and above) with Asxl1 and Asxl2 transcripts, respectively.
Rpl39l was even more distinct on the unfolding plot and was in close proximity to testis; in multiple databases for different species (e.g. Genecards, Mouse Genome Informatics) this gene was indeed described to be testis-specific.
Importantly, DNA introduced into the mouse genome by this method results in establishment of the transgene in the germ line.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com