Genomic sequences were also retrieved from the mouse genome sequence as reported in the NCBI 37 July 2007 assembly (UCSC Genome Browser).
Genomic coordinates of extended human gene loci were transferred to the mouse genome sequence using the UCSC Lift Genome Annotations tool (http://genome.ucsc.edu/cgi-bin/hgLiftOver).
Amplified DNA was then sequenced and blasted against the mouse genome sequence to detect off-target effects.
The availability of the mouse genome sequence has shifted gene trapping from a gene discovery platform to a high‐throughput mutagenesis platform.
Mice have become a preferred model system for bone research because of their genetic and pathophysiological similarities to humans: a relatively short reproductive period, leading to relatively low cost of maintenance and the availability of the entire mouse genome sequence information.
Sequence analysis is at present opening a second principal dimension beyond the databases of species-specific prototype DNA sequences ("the" human genome sequence vs. "the" mouse genome sequence, etc).: In addition to what all creatures of a species have in common, we tackle the problem of what is substantial in the genetic diversity among the members of a population.
While it is true that any stretch of DNA that belongs to all and only humans would be among those differences found by comparing a single human genome sequence to a single nonhuman primate or mouse genome sequence, any "uniquely human" differences could not be distinguished from the others without extensive infra- and inter-specific population studies which are not part of the HGP.
The mouse Mup gene cluster is poorly annotated with repetitive nomenclature in the mouse genome sequence [20].
Primers to genotype the CPD1 mice were generated by testing various primers designed from the mouse genome sequence on the Ensembl database (Mouse Genome Sequencing Consortium).
The locations of restriction enzyme sites at the Evi32 locus based on the published C57BL/6J mouse genome sequence are shown in Figure 1b.
They applied their method (BayesMiRNAfind) to the forward strand of the mouse genome sequence and presented results for different score cutoffs.
