The mouse genome includes caspase-1, -11, and -12, whereas the human and chimp genomes contain caspase-1, -4, -5, and -12, with caspase-12 typically inactivated in the human genome.
Moreover, we generated isoform signatures for all gene isoforms in the human and mouse genome, including both known and computationally predicted ones.
In QSCs, nearly 50% of annotated genes in the mouse genome, including nearly all housekeeping genes and cell-cycle genes, were marked with H3K4me3 at their TSSs.
For ectopic expression, several techniques can be used to introduce novel genetic sequences into the mouse genome, including homologous recombination into a specific locus in the mouse genome, or random integration of transgenes through injection into an embryo.
The International Mouse Phenotyping Consortium is coordinating efforts to phenotype single gene knockouts covering the entire mouse genome, including characterising developmental defects for those knockout lines that prove to be embryonic lethal.
Gene targeting in embryonic stem (ES) cells allows virtually unrestricted manipulation of the mouse genome, including the engineering of large segmental aneuploidies (Liu et al, 1998; Ramirez-Solis et al, 1995) and many pathologies relevant to human disease can be induced in the mouse using environmental, pharmacological or genetic sensitization protocols.
The sequencing of several mouse genomes, including that of the widely used C57BL/6 strain, motivated the development of a resource to elucidate gene function.
In contrast to the evidence from human [ 1] and bank vole, annotations of various rodent mt genome sequences in GenBank (including the reference mouse mt genome) include complete TAG stop codons in the CDS of both nad1 and nad2 that overlap the downstream trnI and trnW by 2 bp (AG) in each case (Fig. 1).
A current listing from the Mouse Genome Database includes 251 genetically determined mouse ocular phenotypes, a number that increases daily.
A similar but unique pattern of homologues is present in the mouse genome, which includes ten genes encoding GH18 family members.
Since the Mouse Genome 430 2.0 includes some 'sibling' probe sets that target mRNAs associated with the same gene, we limited redundancy in our results by considering only the most significantly altered probe set associated with a given gene (i.e., the probe set with the lowest p value).
