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More extensive gene expression analysis, using whole mouse genome expression arrays, will further identify potential downstream targets of PPARγ in trophoblast differentiation.
We performed microarray analysis to compare the expression profiles of the Atrx Foxg1Cre and control telencephalon at embryonic day 13.5 (E13.5) (n = 3 pairs) using an Affymetrix mouse genome expression array representing approximately 39,000 transcripts [ 21].
We also considered data sets from the villous epithelia of mice treated with recombinant RANKL performed on Affymetrix mouse gene 1.0 ST expression arrays and in vitro RANKL-stimulated small intestinal organoids performed on Agilent 4 × 44 K whole mouse genome expression arrays (GSE38785).
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Utilizing Affymetrix whole mouse genome gene expression microarrays, we collected gene expression data from viral (v -Abl kinase-transformed pre-B cell lines that were generated from multiple WT mice expressing an Eμ-Bcl2 transgene.
RNA expression data were generated using Affymetrix Mouse Genome 430 2.0 expression microarrays and analyzed using the "affy" and "limma" packages of the Bioconductor suite of microarray analysis tools available for the R statistical environment [ 83- 86].
Note that OCT4 (POU5F1 − Mouse Genome Informatics) expression is not yet restricted to the ICM in these early blastocysts of either genotype.
In addition, when a similar to the HTM transcriptome map was established for the mouse genome, the expression patterns were found to be conserved to that of the human genome [ 28, 29].
We also found that ectopic mouse Hoxb4 regulates ephrin A5 (Efna5 - Mouse Genome Informatics) expression in a context-dependent manner: downregulating it in dorsal r1 but upregulating it in more posterior hindbrain regions (supplementary material Fig. S2M-T).
Gene expression data sets were selected from the GEO database based on the following three criteria: (i) cell type studied; (ii) chip platform (Affymetrix mouse genome MOE430 2.0 expression arrays) and (iii) availability of raw data (.cel).cel
The biotinylated cRNA targets were then purified, fragmented, and hybridized to GeneChip mouse genome 430 2.0 expression arrays (Affymetrix, Santa Clara, CA) to interrogate the abundance of 39,000 possible transcripts in each sample.
Gene expression data were generated using Illumina's Mouse whole genome expression BeadChips (MouseRef-8-v1 Expression BeadChip).
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