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We validate ESPRIT based on artificially fragmented human genome, on improvements between early and recent mouse genome assemblies.
We report here a genome wide analysis of the large tandem repeats (TR) found in the mouse genome assemblies.
The human and mouse genome assemblies are in the "finished" phases combining both clone-based and whole-genome shotgun strategies [ 7, 28].
Human and mouse genome assemblies (versions hg12 and mm2, respectively) and mappings of GenBank and RefSeq cDNA sequences to the assemblies were retrieved from the UCSC Genome Browser Database [ 26].
We developed a validation framework based on an artificially fragmented human genome, improvements between early and recent mouse genome assemblies, comparison with experimentally validated sequences from GenBank, and phylogenetic analyses.
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We evaluated the expression of 14 222 genes among the studied tissues and for 12 331 genes we were able to assign a genomic position according to the NCBI mouse genome assembly (build 32, mapping 19 684 known LocusLink genes).
This allowed us to avoid any potential discrepancy caused by varying genomic distributions in earlier versions of the mouse genome assembly.
On the basis of a genomic contig (NT_026437) of a more recent mouse genome assembly (build 38), Wheeler et al. (2012) also published the chromosomal coordinates of the mouse RNases, which is consistent with our results.
The hidden Markov models were constructed using HMMer [ 52] from the coding sequences of the manually curated multiple sequence alignment of the mouse and rat Abp genomic DNA alignments (including novel predictions from the mm8 mouse genome assembly).
We mapped the genomic locations of all the L1 elements with the UCSC mouse genome assembly (March 2006, NCBI Build 35, version mm8) using BLAT analysis, with data accuracy greater than 0.98.
Mouse genome assembly GRCm37 (NCBI; UCSC mm9 annotation) was used originally.
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