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The structure of the mouse gene was already known.
As I once wrote in a book called "The Mansion of Happiness," a study of the history of life and death, a mouse gene was the first gene ever cloned; the mouse genome was the first genome decoded.
In 28 cases, the yeast gene was essential, and in 24 of these families (86%), the mouse gene was also essential.
The mouse gene was also responsive to IBMX at the early time points, whereas these effects were only observed at days 1 and 3 in human cells.
Each mouse gene was queried to determine whether it met these criteria for conserved microsynteny.
Briefly, by starting at each gene along each mouse chromosome, each referred mouse gene was used as a starting point for a locus.
Similar(52)
The transcript and exon data of mouse genes were also obtained from Ensembl.
Sequence similarities, domain organization of the deduced peptide, and expression analysis allow us to conclude that this newly cloned mouse gene is Muc6, i.e., the mouse ortholog of human MUC6.
In addition, the mouse gene is expressed exclusively in taste cells, and variations in the gene sequence distinguish taster mice from nontaster mice.
Primers used for amplifying 3'-UTR of each mouse gene were listed in Table S5.
If a mouse gene is expressed at a given stage, we assumed the same activity of the human homologue (Figure 8).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com