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However, the rapidly accumulating datasets will help complete a mouse gene annotation.
The results of this study will contribute to updating mouse gene annotation through the identification of specific tissue-enriched novel transcripts and novel exons.
For our study, we downloaded the most recent GO files and mouse gene annotation lists from the GO website (January, 2009).
After converting the mouse gene annotation to human annotation, 135 genes were common to the 282 genes identified with the co-expression analysis (Additional file 10).
Due to the fact that a gene may contain one or more alias, using only the gene symbols that were included in the mouse gene annotation file would not be sufficient.
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Human gene annotations were converted to mouse gene annotations using the NCBI HomoloGene database.
Therefore, we performed our analyses according to the human and mouse gene annotations separately.
To estimate the sensitivity of Dissect, we initially prepared wild-type transcriptome datasets without any structural alterations using the Known Genes mouse gene structure annotation database (Hsu et al., 2006) and modified extracted wild-type transcript sequences according to various structural alteration scenarios.
(A) Gene structures of RNASEH2C and KAT5 based on mouse RefSeq gene annotation in UCSC genome browser.
For the fruit fly and mouse studies, gene annotation lists were downloaded from http://genome.ewha.ac.kr/ECgene/, in which only transcripts with high quality annotations (confidence high group) were selected.
Mouse gene ontology annotations and homology relationships were obtained from the MGI [33].
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