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NSCs derived from the dissociation of mouse forebrain samples were suspended in culture medium.
The first column is the time-point during T. gondii infection for the mouse forebrain samples used for RNA extraction.
To determine parasite burden in the mouse forebrain samples, a standard curve was generated using a genomic DNA preparation of known parasite numbers.
Therefore to maximize parasite transcripts as well as to compare the same host tissue during acute and chronic infection, we chose to collect mouse forebrain samples at 10 and 28 days post-infection as well as uninfected mice.
Since T. gondii was not purified from the forebrains as a means to rapidly process the samples and preserve the interactome, uninfected mouse forebrain samples were mapped to the T. gondii TGME49 reference to determine the extent of false positive reads.
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The fourth column is the total number of paired-end reads from each mouse forebrain sample that mapped to the T. gondii genomic reference file.
Additional resources include: the mouse forebrain sample using affinity-based IMAC/C18 enrichment [ 54], the human mitotic phosphoproteome based on SCX chromatography, IMAC, and TiO2 enrichment [ 55], the mouse liver and Drosophila embryo [ 30].
Every fourth slice was collected for a total of 10 slices from each basal forebrain sample.
We also analysed the acetylation status of FOXO1 in the forebrain samples of Tau-Cre; Rosa26 Sirt1-WT (Sw) mice that received intracerebroventricular (i.c.v).
Open image in new window Figure 2 Reduced cell proliferation and neurogenesis in aged mouse forebrain neurogenic zone.
RNA was extracted from mouse forebrain using Trizol.
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