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L929 mouse fibroblasts were cultured on the nanoyarn scaffolds.
NIH3T3 mouse fibroblasts were cultured in DMEM medium (nacalai tesque), supplemented with 10% fetal bovine serum and 100 U/mL penicillin-streptomycin.
Whether TGF-beta1 directly influences Wnt-4 expression in fetal or postnatal fibroblasts has not been examined.Primary fetal and postnatal mouse fibroblasts were stimulated with TGF-beta1 and Wnt-4 expression quantitated by real-time polymerase chain reaction.
The 3T3 mouse fibroblasts were successfully confined into circular, 100 μm2 cell-adhesive areas that were patterned inside a cell-resistant layer.
However, attachment and proliferation of BALB/3T3 mouse fibroblasts were significantly improved on films made of the RGD-modified silk proteins.
Primary mouse fibroblasts were isolated from differential platting of day 1 mouse hearts and cultured as previously described [50].
Similar(6)
L929 mouse fibroblasts are then cultured onto these patterned surfaces for durations of 24 h and 48 h, respectively.
Furthermore, the influence of the thiol groups on the viability of L-929 mouse fibroblasts was evaluated.
In addition, the cell viability of mouse fibroblasts was better for PGS samples with a higher conversion.
On the other hand, it was reported that the spreading of mouse fibroblasts was more supported on hydrophilic than on the hydrophobic electrospun surfaces (Bhattarai et al. 2006).
These studies were all performed on mouse fibroblasts being converted into heart cells, but they did not address the question whether human fibroblasts would behave the same way.
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