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Furthermore, the influence of the thiol groups on the viability of L-929 mouse fibroblasts was evaluated.
We found that the repair capacity for uracil-DNA in wild type mouse fibroblasts was very strong, whereas the repair capacity for THF-DNA, although strong, was slightly weaker.
In this work, the migratory behaviour of mouse fibroblasts was investigated on poly ethylene glycol) hydrogels (PEG) where only one triggering signal was present at a time, i.e. either topographic structures or a stiffness contrast.
The cell viability and adhesion of L929 mouse fibroblasts was investigated using two different colorimetric assays: (i) 2- 4,5-dimethyl-2-thiazolyl -3,5-diphenyl-2H-tetrazolium bromide (MTT), and (ii) lactate dehydrogenase (LDH).
Antiproliferative activity on CCL-8 (mouse sarcoma), MDA-MB-435s (human melanoma), MES-SA (human uterus sarcoma), MCF-7 (human breast adenocarcinoma), HT-1080 (human fibrosarcoma), MG-22A (mouse hepatoma) tumor cell lines, and normal cell line NIH 3T3 (mouse fibroblasts) was studied.
The cytotoxicity of the homopolymers, oligomers, and polyplexes toward human fibroblasts and 3T3 mouse fibroblasts was evaluated using the MTT and AlamarBlue™ assays, proving that formulations could be made with toxicity as low as low molecular weight linear poly (dimethylaminoethyl methacrylate) (PDMAEMA).
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L929 mouse fibroblasts were cultured on the nanoyarn scaffolds.
NIH3T3 mouse fibroblasts were cultured in DMEM medium (nacalai tesque), supplemented with 10% fetal bovine serum and 100 U/mL penicillin-streptomycin.
L929 mouse fibroblasts are then cultured onto these patterned surfaces for durations of 24 h and 48 h, respectively.
However, attachment and proliferation of BALB/3T3 mouse fibroblasts were significantly improved on films made of the RGD-modified silk proteins.
Whether TGF-beta1 directly influences Wnt-4 expression in fetal or postnatal fibroblasts has not been examined.Primary fetal and postnatal mouse fibroblasts were stimulated with TGF-beta1 and Wnt-4 expression quantitated by real-time polymerase chain reaction.
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