Live-cell analysis of endogenous β-actin mRNA in mouse fibroblasts reveals that mRNA polarization has a half- life of ~16 min and is cross-correlated with directed cell migration.
Analysis of CBP and p300 mutant mouse fibroblasts reveals CBP/p300 are together chiefly responsible for the global acetylation of histone H3 residues K18 and K27, and contribute to other locus-specific histone acetylation events.
In vitro test using L929 mouse fibroblast revealed that, the number of cells proliferated on PICP-treated samples was higher than that on untreated samples.
Our studies on VEGFA regulation revealed that XBP-1(S), a UPR-inducible transcription factor, bound to two regions on the VEGFA promoter, and analysis of XBP-1 null mouse embryonic fibroblasts revealed that it contributes to VEGFA expression in response to ER stress.
The promoter of p16 is suggested to locate around the transcription-start-site (TSS), and a previous study using mouse embryonic fibroblasts revealed that H3K27 methylation status around this region is altered by OIS.
Our previous data obtained from transcriptome analysis using THOC5 depleted bone marrow macrophages, or mouse embryonic fibroblasts revealed that several chemokine/growth factor genes or metalloprotease genes were THOC5 dependent [ 17, 18].
The microarray conducted in RasCA-transformed mouse embryonic fibroblasts revealed that many genes encoding DNA-associated proteins (involved in DNA replication and DNA repair) are upregulated as well [ 30].
Activated endogenous ERK was visualized in mouse embryo fibroblasts, revealing greater activation in the nucleus, perinuclear regions, and especially the nucleoli.
Our recent data obtained from transcriptome analysis using serum induced mRNAs in mouse embryonic fibroblasts reveal that upon stimulation with serum, 94 genes were upregulated more than 4-fold within 1 h in the presence of THOC5.
Indirect cytotoxicity evaluation of the fiber mats with mouse fibroblasts (L929) revealed that the materials were non-toxic and did not release substances harmful to living cells.
