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Mouse fibroblasts behaved similarly, but only when they senesced in 3%, not 20%, O2 (Fig. 5D,E).
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The HSPB1R127L fibroblasts behaved largely as control cells during canavanine treatment.
Collectively, IPF fibroblasts behave more like tumor cells, indicating that they have undergone epigenetic changes, or have transformed somehow from a normal lung fibroblast to a disease phenotype.
In mouse fibroblasts synchronised by mitotic selection, TS behaved as a cell cycle-dependent enzyme with mRNA levels undergoing a 5- to 10-fold increase as cells progress from G1- to S-phase (Nagarajan and Johnson, 1989).
Interestingly, mouse embryonic fibroblasts (MEFs) behaved as most of the cancer cell lines showing HMGA1 expression in both the compartments.
These studies were all performed on mouse fibroblasts being converted into heart cells, but they did not address the question whether human fibroblasts would behave the same way.
The CDs in vitro toxicity was studied on standard mouse fibroblasts (NIH/3T3).
A similar finding was described previously when comparing cultured wildtype mouse fibroblasts to fibroblasts from the rd16 mouse, which expresses a microtubule-binding deficient variant of CEP29026.
Mouse fibroblasts (L929) were seeded onto the non-irradiated and irradiated surfaces to evaluate cell attachment capabilities.
In vitro cytotoxicity tests showed that these polyamides are nontoxic towards mouse fibroblasts and have great potential in biomedical applications.
Adams, R. L. The relationship between synthesis and methylation of DNA in mouse fibroblasts.
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