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Mouse fetuses are extensively studied to determine the roles of genes in development and congenital abnormalities.
In this paper, results from first studies on mouse fetuses are presented.
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Mouse fetuses were harvested from 14-day pregnant Cavin-3 KO and wild-type mice.
Tissue from both E13.5 and E16.5 mouse fetuses was tried; the latter was selected, as development of the forebrain occurs at a later embryonic time point than that of the spinal cord.
Lung explants from gestation day 13 or 14 Sprague-Dawley rat fetuses or gestation day 12 Tie2-LacZ mouse fetuses were cultured in DMEM/F-12 supplemented with 2% stripped serum on Whatman polycarbonate filter supports and cultured at fetal P o2.
Expression of HIF-1alpha and -beta in the mouse fetus was highest in the brain followed by heart, kidney, lung, and liver.
Since a mouse fetus is very small, an anatomical study requires troublesome embedding, staining and sectioning that require high skills.
Since a mouse fetus is mostly made of soft tissues, only poor contrast is obtained by absorption-based X-ray CT of a native fetus.
The CF-1 male mouse fetus is thus between 25,000 and 100,000 times more sensitive to ethinylestradiol relative to the CD-SD rat.
Mouse fetus was cut in two and washed with PBS once followed by incubation at 37oC for 30 min with X-gal staining buffer (5 mM K4Fe CN 6⋅3H2O, 5 mM K3Fe CN 6, 20 mM MgCl2, 1 mg/ml X-gal).
To induce the experimental testicular teratoma, male gonadal ridges from 12-day-old 129/SvJ-mouse fetuses were grafted into the testes of adult mice for 1 12 weeks.
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