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If a mouse failed to find the platform within 60 s, it was manually guided towards it and allowed to remain there for 10 s.
If the mouse failed to move to the opposite chamber after 2 s of US presentation (hence after a total of 7 s), the trial was terminated and the gate closed (no response).
Although this was hailed as support for a link between hippocampal neurogenesis and depression, the behavioural test had more to do with anxiety than depression, and a further study (from the same group) using a different test (forced swim) and a different strain of mouse failed to replicate it [14].
If the mouse failed to reach the wooden platform, it was assigned a score of "fail".
Cells were considered nontumorigenic if mouse failed to form tumour within 4 months of injection.
By contrast, antibodies of irrelevant specificity in the mouse failed to accumulate.
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Solvent-only-treated mice failed to develop elevated TEWL values above the physiological threshold (A).
These mice failed to suckle and died a day or so after birth.
We found that E-cadherin conditional knockout mice failed to survive, dying within the first 24 hours of birth.
When compared to platelets from wild-type littermates, platelets from α2-null mice failed to adhere to type I collagen under either static or shear-stress conditions.
The MSCs isolated from mTR−/− mice failed to differentiate into adipocytes and chondrocytes, even at early passages, whereas WT MSCs were capable of differentiation.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com