Sentence examples for mouse eyecups were from inspiring English sources

Briefly, mouse eyecups were prepared from freshly dissected eyes.

The overall melatonin levels (averaged over all time points) in the transgenic eyecups were not significantly different from those in the eyecups from the wild-type siblings (Fig. 3B) or XOP-XCLΔQ-GFP eyecups (Fig. 3B, compare to 3A).

The eyecups were washed in PBS followed by 30% sucrose in the same buffer overnight.

Eyecups were prepared by removing the cornea along the limbus and then the iris.

Eyecups were then cultured in a perfusion chamber in constant darkness (DD) as previously described.

After soaking in 30% sucrose, the eyecups were shock frozen in liquid nitrogen.

Eyecups were superfused for 60 min in the dark before the start of electrical recording.

RPE-sclerochoroidal eyecups were set aside for the primary culture of RPE cells.

Eyecups were vertically sectioned at 12 µm on a cryostat and collected on poly-L-lysine-coated slides.

Following fixation, the eyecups were rinsed in 0.1 M PB and cryoprotected in graded sucrose solutions (10%, 20% and 30%).

The agarose-embedded eyecups were placed in Tissue Tek® O.C.T. compound (Sakura Kinetek U.S.A. Inc., Torrance, CA) and frozen in liquid nitrogen.