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However, in the mouse eye, the increased depth of focus provided by a small diameter beam exacerbates the problem of multiple back reflections from multiple retinal layers.
It was reported that in one mouse eye, the hyperopic spherical equivalent was reduced by 7 D in the anesthetized eye [ 13].
Chx10 is restricted to the inner nuclear layer (INL) in the mature retina, though in the developing mouse eye, the Chx10 transcript is confined to the anterior optic vesicle and all neuroblasts of the optic cup.
In that case, taking into account the measured chromatic aberration of the mouse eye, the average refraction at the disk (central retinal artery) for 514 nm was calculated to be 13.4 D for a 2 mm pupil, or 15.3 D for a 0.8 mm pupil.
To estimate the true refraction of the mouse eye, the average refraction at the outer retina was calculated to be quite myopic at −15 D for a wavelength of 514 nm (that is, in which the chromatic aberration has been taken into account).
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Incorporating TCA correction for the eye's chromatic aberrations will be particularly important in mouse eyes (the most common animal model used in biomedical research) where the chromatic dispersion is about 10 times greater than in humans [ 31], and where it is desirable to have excellent image alignment when multiple fluorescent labels are present in the retina [ 32, 33].
The ratio of total eye power between the mouse eye and the human eye is ~9.6, thus estimates of the mouse eye LCA would be 9.6 times the human LCA.
Based on higher order aberrations, the mouse eye and the human eye MTF drop from the diffraction-limited MTF in a similar way, and the human eye modulation transfer is higher than that of the mouse eye for all frequencies.
Heterotransplantation of retinoblastoma cells into the anterior chamber of the nude mouse eye and the subsequent growth of small tumour masses has provided a model for evaluation of various therapeutic modalities.
RNA ISH analysis revealed a number (n = 50) of regional miRNAs that stained specifically the uveal tunics of the mouse eye in particular the iris and the ciliary body (CB) (Additional file 4; Figure 7A).
The bite bar itself could be translated in two dimensions to align the mouse eye pupil to the center of rotation of the stage.
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