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Mouse eye sections were incubated in PBS diluted antibodies.
Remtulla and Hallett developed a homogeneous schematic eye from measuring frozen mouse eye sections [ 1].
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Immunohistochemistry was performed at room temperature in the same way as it was done with the mice eye sections.
Eye sections from WT and M 2 mutant mice (Chrm2+/+, Chrm2+/−, Chrm2−/−; n=6 eyes from each strain) were prepared as above for the staining of sections with antibodies specific for M1 M5.
To analyze RGC survival, retinal sections were examined for Rbpms immunoreactivity from ora serrata to ora serrata through the central region of the mouse eye.
The mouse eye has been used as a model for studies on the microanatomy of the outflow pathways but most of what is known comes from histological sections.
Close-up photo of mouse eye showing injected cells (green).
The antibodies listed above in the immunocytochemistry section were also used on eye sections together with a cocktail of antibodies generated against human-specific markers (HSM) to identify human cells (the Oka blood group antigen, mouse TRA-1-85, 1∶10 (a kind gift from Peter Andrews, University of Sheffield, Sheffield, UK) together with mouse human nuclear antigen, 1∶1000, Millipore).
Next, we immunostained human eye sections using the same antibody.
*Mouse eye f and z are values for the adult mouse eye [ 2].
Tangential eye sections did not reveal any structural defects in ommatidial arrangement.
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