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In both human and mouse, expression breadths are extremely strongly correlated with expression levels [Pearson's correlation r =0.81 and 0.82 for human and mouse, respectively, P< 2.2 × 10 16 for both comparisons (16, 17)].
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This relationship appears to be lineage-specific; in the mouse data, there was no significant relationship between synonymous rates and expression breadths (Table 3).
Although gene expression level correlates closely with expression breadth, CUBs are comparable among different expression breadths.
According to Figure 3, CUB does not change gradually along with expression breadth and CUB does not even tend to be different between different expression breadths.
Indeed, the correlation between DNA methylation breadth and gene expression breadth was about twice as stronger as that between promoter CpG contents and gene expression breadths (Supplementary Material, Table S4).
Consequently, promoter CpG content was considered as a proxy for gene expression breadths (47– 47).
One interesting species-specific difference is the relationship between gene expression breadths and synonymous evolutionary rates.
Evolutionary rates are also negatively associated with gene expression breadths (11– 11).
In comparison, in primates, expression breadths and synonymous rates exhibit significant relations.
In EST data, the expression breadths clearly peaked at the value of 18.
This is interesting, as these two genes have very different expression breadths.
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