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A similar procedure might be tried in people, he said, if the mouse experiments are promising.
The mouse experiments are "just what needs to be done," Fein says, although she cautions that a mouse lacking oxytocin "is a long way from being a comprehensive model of a human disorder".
Although these two numbers are not directly comparable since the mouse experiments are more controlled than the human-chimpanzee comparison and thus have more power to detect difference, the extent of expression differences induced in the liver by a change in diet is impressive.
As mouse experiments are generally better controlled than human studies and there is less variation caused by genotypic and environmental factors in the mouse, Mus musculus data was chosen over Homo sapiens to reduce noise.
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The mouse experiments were a run-up to monkey experiments.
Mouse experiments were approved by the Academic Medical Center Animal Use and Welfare Committee (DIX16AB).
All mouse experiments were performed under a Baylor College of Medicine Institutional Animal Care and Use Committee (IACUC) approved protocol.
But while mouse experiments were promising, the results in human trials were disappointing, Klenk cautions.
"The magnitude by which the low-[fiber] diet depletes the microbiome in the mouse experiments is startling".
The absorbed tumour doses of the mouse experiments were extrapolated from the sphere model doses which were calculated by using the software OLINDA (OLINDA/EXM1.0, Vanderbilt University, Nashville, TN, USA).
All mouse experiments were done in accordance with protocols approved by the Institutional Animal Care and Use Committee of MSKCC and followed National Institutes of Health (NIH) guidelines for animal welfare.
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