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Here, our analysis of distinct human and mouse epithelial subtypes has highlighted potential miRNA networks responsible for governing lineage commitment and differentiation in mammary tissue.
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The predicted miRNA mRNA relationships were found to be highly conserved between mouse and human epithelial subtypes.
Distinct epithelial subtypes have been prospectively isolated from both mouse [ 2- 5] and human mammary glands [ 6- 10].
The high degree of conservation between analogous epithelial subtypes across species supports the use of mice as a model system to study normal mammary gland development and oncogenesis.
Among epithelial subtypes, UPR genes were most enriched in AT1/AT2 alveolar cells, and in Sox2hi conducting airway epithelial cells (Fig. 6a and Supplementary Data 6).
Four major epithelial subtypes or transcriptional states were revealed by gene expression analysis of selected populations of single cells.
However, the molecular mechanisms that generate these epithelial subtypes are not well understood.
In the mature thymus, thymocyte maturation depends on interactions with different thymic epithelial subtypes in a three-dimensional thymic architecture.
Different epithelial subtypes have been defined: serous (50 70%), endometrioid (10 25%), clear cell (10%), and mucinous (5%).
Luminal-A and basal breast cancers correspond to two very distinct epithelial subtypes.
In the Drop-seq data, 20 out of 1809 epithelial cells co-expressed Sftpc, Ager, and Scgb1a1 RNA, representing 0.2% of total lung cells in the Drop-seq data; these cells clustered in the AT1/AT2 epithelial subtype (Supplementary Figure 7g).
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