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In this study, we systematically screened 297 cellular miRNAs from human and mouse epithelial cells and identified five inhibitory miRNAs that efficiently inhibited influenza virus replication in vitro and in vivo.
IL-10 in the lumen may be detected by DCs that protrude their dendrites through the epithelial cell layer to sample antigens [41], [51] and mouse epithelial cells were proven to express the IL-10R [52].
The NF-κB pathway is important for lung inflammation in mouse epithelial cells, and individuals with ARDS have increased activation of NF-κB in alveolar macrophages when compared to controls [14], [15].
In contrast, AvrA is able to attenuate the key proinflammatory NF-κB transcription factor [14], [17], activate the β-catenin transcription factor [27], [36], and inhibit cell apoptosis in mouse epithelial cells [17].
Mouse epithelial cells were scraped and lysed in lysis buffer (1% Triton X-100, 150 mM NaCl, 10 mM Tris pH 7.4, 1 mM EDTA, 1 mM EGTA pH 8.0, 0.2 mM sodium ortho-vanadate, protease inhibitor cocktail) and protein concentration measured.
In direct co-cultures (Fig. 5B E), mouse epithelial cells were mixed with human PRE or SEN XRA or REP) fibroblasts (strains WI-38 or hBF) [15] (Fig. 5B,C), or mouse PRE or SEN XRA or OXI) fibroblasts (MEFs or mBFs) (Fig. 5D,E).
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At a distance of 50 µm from the mouse epithelial cell surface, the inner attached mucus is converted into the outer mucus and expands in volume.
However, the over-expression was also observed in surgically resected normal tissue and mouse epithelial cell layers 4 hours after resection (Figure 6).
Expression profiles of the mouse epithelial cell layers at 0 or 4 hours after resection revealed that mouse Zeb1, Zeb2, Vim, and Fn were induced 4 hours after resection (Figure 6B, lower).
Lineage− mammary epithelial cells were obtained after removing the CD45+, Ter119+, and CD31+ cells using the EasySep mouse epithelial cell enrichment kit (StemCell Technologies).
TC-1, a mouse epithelial cell line transformed with the oncogenes Ras, HPV16 E6, and E7, was a kind gift from Dr. T-C.
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