This gene was not found differentially expressed in mouse, rat and human epididymal transcriptome.
In their study Jelinsky et al, [ 20] whom made a detailed comparison on both the mouse and rat epididymal transcriptomes were strongly limited due to the identification of orthologous sequences present on arrays.
In the epididymal transcriptome of Aa-infected mice, the up-regulated genes were Pcx (FC = 2.82), fatty acid synthase (Fasn, FC = 1.81), Scd2 (FC = 2.15), and Elovl6 (FC = 3.21).
This study was designed to identify the complement of microRNAs (miRNAs) that are expressed within the mouse epididymal epithelial cells and the maturing populations of spermatozoa.
Each siRNA with HongrES1 complementary DNA was co-transfected into the mouse epididymal epithelial cell line (PC1), which had no endogenous HongrES1 expression.
From this point of view, the sequencing data presented in this report might include the majority of the human epididymal transcriptome with relatively higher expression level.
Biological activity was determined by glycerol release from mouse epididymal adipocytes, as previously described (Hirai et al, 1998).
For histology, mouse epididymal fat pad from Tgm2+/+ and Tgm2−/− mice were fixed in 10% neutral-buffered formalin and paraffin embedded and stained with eosin and hematoxylin.
Total RNA was extracted from mice epididymal fat, liver and brain using RNeasy® Lipid Tissue Mini kit (Qiagen).
