The study of keratinocyte differentiation markers as a function of age shows that K1 expression extended to some hair follicles of knockout mice, while K6 was aberrantly expressed in the epidermis.
Here, by in situ hybridization we characterize the expression pattern of miR-203 after the induction of wound in mouse epidermis, showing that its expression is downregulated in the highly proliferating keratinocytes of the 'migrating tongue', whereas it is strongly expressed in the differentiating cells of the skin outside the wound.
In mouse epidermis, however, lipin-3 expression is relatively abundant (CT: 25∼26), while lipin-1 and -2 expression levels are in similar ranges (CT: 28∼29).
51 Using RT-PCR, DGAT-1 and 2 mRNA can be detected in adult mouse epidermis but DGAT-2 expression is much more robust than DGAT-1 (Table 1 ).
Additionally, neither DGAT-1 nor DGAT-2 expression changed following acute barrier disruption by tape-stripping in hairless mouse epidermis, indicating that basal DGAT expression suffices to meet the permeability barrier requirement in adult mice.
As predicted from their predominant localization in the basal layer, loss of Dsc3 and Dsg3 gene expression in the mouse epidermis causes impaired cell cell adhesion, leading to intra-epidermal blistering between the basal and suprabasal compartment (36, 37).
We have now examined Lrig1 expression and function in mouse epidermis.
Next, we examined the role of p53 on the regulation of UVB-induced lincRNA-p21 expression in vivo in mouse epidermis using K5Cre+/tg p53flox/flox mice.
The elevated expression of K6 in knockout mouse epidermis could be a contributing factor to wrinkle formation and flakiness in skin.
33 In mouse epidermis, AGPAT-3, -4 and -5 have relatively high constitutive expression levels, while AGPAT-1 and -2 have low constitutive expression levels.
