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After the injections, the mouse embryos were reimplanted in females and carried to term.
Mouse embryos were isolated in cold PBS and fixed in 4% paraformaldehyde for 2 3 h, followed by equilibration in 30% sucrose in PBS solution overnight.
Fore and hind limbs from E11 E13 ICR mouse embryos were cultured for six days, either in the bioreactor or in center-well organ culture dishes, fixed, and embedded for histology.
Intact, manually separated blastomeres from wild type and maternal knockout E-cadherin+/− two- to eight-cell stage mouse embryos were used.
The first sign that this is not always true came from experiments in which mouse embryos were engineered to carry two male genomes, or two female genomes.
Mouse embryos were first cultured to very late 2-cell stage and then monitored every hour.
E13.5 Mouse embryos were dissected in Knockout DMEM (Invitrogen).
Mouse embryos were processed for whole-mount X-gal staining.
In situ hybridization: E11.5 mouse embryos were embedded in OCT.
Mouse embryos were isolated at day 10.5 of gestation (E10.5).
Microinjected mouse embryos were transferred into the oviduct of foster females.
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