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Sommer, D. et al. Efficient genome engineering by targeted homologous recombination in mouse embryos using transcription activator-like effector nucleases.
For the first time, vector-flow information has been obtained from the cardiovascular system of in utero mouse embryos using HFU and high-speed, plane-wave imaging techniques.
Here, we demonstrate the feasibility of in utero vector-flow imaging of blood flow in mouse embryos using a high-speed, HFU plane-wave and vector-flow imaging approach capable of sub-ms, full-frame image capture.
In a breakthrough that redefines how life can be created, embryologists working at the University of Cambridge in the UK have grown realistic-looking mouse embryos using only stem cells.
While this manuscript was under preparation, Kim et al. reported generating fully base-edited mouse embryos using BE3 (Kim et al., 2017a).
As reported online yesterday in Nature Cell Biology, Akira Sawa of Johns Hopkins University in Baltimore, Maryland, and colleagues disrupted DISC1 expression in the brains of mouse embryos using a technique called RNA interference.
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Isolation of mRNA from antler tissues or whole E17.5 mouse embryos used the MicroPoly(A Pure mRNA purification kit (Ambion, Austin, Texas).
We would like to thank Brian DeVeale and Derek van der Kooy at the University of Toronto for donating all mouse embryos used in this study.
DNA was isolated from the tail tissue of each mouse embryo, using 50 mM NaOH for 30 minutes at 95°C followed by 1 M Tris buffer, pH 8 at room temperature.
Targeted ES clones were introduced into an 8-cell stage mouse embryo using the VelociMouse method (Poueymirou et al., 2006).
Direct evidence for a role for Spry in angiogenesis comes from a study in which the mouse Spry4 was overexpressed in the developing endothelium of a mouse embryo using an adenoviral vector (Lee et al, 2001).
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