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Using cells from mouse embryos, they produced three mice that were genetically identical, and cleared the way for experiments with larger mammals.
By inserting an extra chromosome into mouse embryos (they used mouse Chromosome 16, a portion of which is analogous to human Chromosome 21), they produced a mouse that exhibited many of the characteristics of Down syndrome.
For example, when introduced into early-stage mouse embryos, they produced chimeric animals that have body tissues containing two different genomes, the team reports today in the journal Cell Stem Cell.
After obtaining such germs from mouse embryos, they separated out two types of cells--epithelial cells and mesenchymal cells--and then recombined them into a new bioengineered tooth germ.
Organ rudiments were microdissected from E11.5 NMRI or CD1 mouse embryos; they were pooled and assigned randomly to control or experimental groups.
In mouse embryos they observed hemorrhages in the forebrain with a leaky BBB for sulfo-NHS-biotin, coupled with a lower vascular density in the hindbrain.
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When these neurogenin 3 cells were taken from an adult mouse and injected into a pancreas removed from a mouse embryo, they developed into beta cells and produced insulin, suggesting that the cells were developing into new beta cells in the injured animal.
When mouse teratocarcinoma cells were transplanted to normal mouse embryo, they had stable differentiation and were incorporated into the tissue [ 26].
Importantly, inducible Pax7 ablation in developing mouse embryos promoted brown fat development.
They grew mouse embryos until they had 1000 cells.
They were looking for genes that affect early development of mouse embryos, so they exposed the embryos to a chemical mutagen and found mutated genes that caused early neural defects.
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