DOI: http://dx.doi.org/10.7554/eLife.07178.002 The successful isolation and in vitro culture of embryonic stem cells (ESC) from mouse embryos have enabled technological breakthroughs and revolutionized our understanding of the molecular mechanisms regulating mammalian development (Evans, 2011).
The results demonstrate that HREM combined with a systematic screening protocol enables more efficient phenotyping of E14.5 mouse embryos than any alternative approach.
Future improvements in imaging should enable us to generate large libraries of digital mouse embryos from fertilisation to implantation that can be interrogated for quantitative information and population behaviour.
Ultramicroscopy is a planar illumination microscopy technique, which enabled us to perform optical sectioning of immunostained, cleared, intact mouse embryos at E9.5 E12.0.
As reported here, the properties of LATE-PCR have enabled us to expand our earlier studies on differential gene expression in blastomeres of preimplantation mouse embryos [ 4, 10, 11].
The scientist, Beatrice Mintz of the Fox Chase Cancer Center in Philadelphia, inserted mouse cancer cells into early mouse embryos.
The altered cells are then introduced into early mouse embryos, which are then implanted in a mouse womb.
After the injections, the mouse embryos were reimplanted in females and carried to term.
Laboratory tests have also demonstrated that ammonium can interfere with the development of mouse embryos.
The first chimeric animals were created by researchers in the 1960s, when experiments with mouse embryos showed they could combine to form a single mouse of normal size.
Using cells from mouse embryos, they produced three mice that were genetically identical, and cleared the way for experiments with larger mammals.
