Surprisingly, these specificities of the mouse dentition appear to be a benefit for experimental studies on odontogenesis: it is very advantageous that the jaw of a mouse embryo allows studying and comparing the formation of a tooth with continuous/unlimited growth (incisor), the origin of a toothless diastema, and successive development of molars with limited growth (Fig. 6c).
DOI: http://dx.doi.org/10.7554/eLife.06489.013 In contrast to mouse, the size of zebrafish embryos allows oxygen to reach tissues independently of cardiovascular-based convection.
To further examine possible mechanisms that lead to the observed strong phenotype in vivo, we established an immortalized 3T3 cell line based on mouse embryonic fibroblasts (MEFs) of floxed embryos, allowing for the inducible homozygous knockout of Dohh.
First, external fertilization and transparency of zebrafish embryos allow better visualization of early blood-related phenotypes than mice, where development occurs in utero.
A detailed staging of individual embryos allows composition of a homogenous sample of embryos at a particular stage of developmental progress (e.g. for quantitative or comparative studies in wild type and mutant mice), or of ranking the embryos in a series of detailed step-by-step developmental stages to trace the dynamics of a specific event.
Briefly, SCG ganglia were harvested from mouse embryos at embryonic day 15, dissociated, and allowed to settle on tissue culture grade plates coated with 0.5 mg/ml poly-ornithine and 10 µg/ml laminin.
The genes we have identified here as markers of the tNP population may now allow the identification of similar progenitors in the mouse embryo and provide an entry point to dissect the genetic circuitry controlling this stage of neural development.
Here we describe an alternative method that allows the generation of clonal mouse embryos harboring a single-copy transgene at a defined genomic location.
Sophisticated experimental tools with high-resolution analysis have allowed us to explore cell lineage restriction within the hindbrain in mouse embryos.
In the case of early mouse embryos, detection of the Sry gene, which is not expressed at those stages, has also allowed us to identify the sex of each embryo.
