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Total RNA was obtained from mouse embryonic tissue or adult mouse organs and reverse transcription carried out as described [ 34].
An initial study on chimeric mice described that Minute cells were also eliminated from mouse embryonic tissue, but mechanistic insight was limited [ 15].
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The objective of this study was to characterize ADNP in mouse embryonic tissues throughout development.
In addition, we show that Math6 is broadly expressed in mouse embryonic tissues and its expression is induced by tissue-specific bHLH genes other than Neurog3.
We show that this alternative splicing takes place in mouse embryonic tissues and that the product of the new isoform is expressed in the embryonic brains of mice and human embryonic stem cells.
Identification of a relatively high fraction of genes commonly regulated by Pax6 between two distinct mouse embryonic tissues suggests that Pax6 participates in similar regulatory events during both lens and forebrain development.
Candidate genes for facial clefts were selected from a variety of resources, including published linkage and association studies on clefts, genome-wide scans, gene-knockout experiments in mice, studies of chromosomal rearrangements in humans, and gene expression analyses in human and mouse embryonic tissues [2], [4], [20], [21], [22], [23].
Otherwise there was generally low-level expression in the mouse embryonic tissues, including the pancreas.
For example, recombination studies with mouse embryonic tissues have demonstrated that early androgen signalling occurs through the stromal compartment.
Differential expression of the mouse Pdcd2 gene was reported in E9.5 parthenogenetic versus androgenetic mouse embryonic tissues of the B6 strain, suggesting it may be paternally imprinted [ 21].
The expression of HOXC11 protein has been reported in mouse embryonic tissues like kidney, prostatic urethra, femur, pelvis and gut [ 6, 24].
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